Biological Characteristics And Biosynthesis Of Exopolysaccharides (Eps) From Cordyceps Militaris Fna5 Using Submerged Fermentation

Biological Characteristics And Biosynthesis Of Exopolysaccharides (Eps) From Cordyceps Militaris Fna5 Using Submerged Fermentation
Nguyen D. Thang, Pham T. Huyen, Bui V. Ngoc, Nguyen P. Nhue, Nguyen V. The, Dang T.T. Duong, Hoang T. Yen, Phi Q. Tien
Institute of Biotechnology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Cau Giay
Asbtract
Cordyceps militaris, a medicinal mushroom, is one of the well-known insect fungi that contain many bioactive compounds such as polysaccharides, cordycepin, adenosine, etc. These compounds show remarkable biological activities, for example, antitumor, immunomodulating, antioxidant, and pro-sexual agent. Thus, the aim of this work is to characterize and optimize the biosynthesis of exopolysaccharides from C. militaris FNA5 strain using submerged fermentation. The strain FNA5 was isolated in the Pu Mat National Park, Nghe An province. Then, ITS region of the strain FNA5 was PCR amplified and used as a molecular marker for identification of fungal species. The output showed that this strain belongs to the genus Cordyceps militaris. The optimal physical and nutritional conditions for production of exopolysaccharides were investigated by individually varying one variable at a time. The suitable physical conditions were determined as follows: pH 6, temperature 25o C, rate of inoculum 3% (v/v), inoculum age 84h, incubation time 15 days. The optimal medium proportion was 3% glucose, 1% peptone, 0.05 % K2HPO4 , 0.07 % KH2PO4 , and 0.05% MgSO4 .7 H2O. At such conditions the maximum yield of exopolysaccharides (EPS) was achieved as 2031.241 mg l -1 . These fingdings indicated that newly developed medium could be used in the industrial production of EPS and other bioactive substances from the FNA5 strain contributing to promote public health in Vietnam.